Group B Streptococcus (GBS) colonization of mother during pregnancy is an important risk factor for early onset neonatal sepsis (EONS). Use of prophylactic intrapartum antibiotics in colonized mother, is associated with reduced early onset disease (EOD) in neonates. A cross sectional analytical study was conducted at the department of Microbiology of Sir Salimullah Medical College, Dhaka. Total 107 of each vaginal and vaginorectal swabs was collected from pregnant women at 35-37 weeks of pregnancy. Vaginal and vaginorectal samples were tested by standard culture technique using Todd-Hewitt broth, blood agar media. All GBS isolates were subjected to antimicrobial susceptibility testing by disc diffusion method. Among 107 study population 11(10.28%) were identified as GBS carriers. All (100%) isolates were sensitive to penicillin, ampicillin, ceftriaxone, cefepime and vancomycin. While 9.09%, 27.27% and 36.36%, isolates were resistant to clindamycin, erythromycin and chloramphenicol respectively.