
Sahar Moghbelinejad
Associated Professor at Qazvin university of Medical science, IranPresentation Title:
Radio sensitivity effect of metformin on breast cancer cells through the expression modulation of miR-21-5p/SESN1 axis
Abstract
The anti-cancer effect of metformin has been shown in various studies, in this research we showed radio sensitivity effect of metformin on breast cancer cells. The expression rate of miR-21-5p and SESN1 gene in radio resistant and control cells was checked by Quantitative Real-time PCR (qRTPCR). The rate of cell proliferation and cell death was checked by CCK-8 assay and flow cytometry. The Western blot method was also used to confirm the expression of some genes. Our results showed that the expression of miR-21-5p was up regulated in radiation-resistant cancer cells, while the expression of SESN1 was down regulated. Metformin enhanced the radio sensitivity of breast cancer cells in a dose and time-dependent manner. Also, metformin treatment decreased the expression of miR-21-5p and increased the expression of SESN1. In conclusion, based on this research results, metformin enhanced the radio sensitivity of breast cancer cells via modulating the expression of miR-21-5p and SESN1.
Biography
I am Dr Sahar Moghbelinejad, Associated Prof. Of Medical Genetics. My research field is study of molecular basis of cancers specially Breast cancer. I have various publications in this area.